Utilization and Outcome of Abnormal 2PN Human Embryos

Abstract

Abstract OBJECTIVE There are not a few embryos derived from abnormal 2PN zygotes or embryo. For patients with non-normal available embryos, whether such embryos are discarded or continue to be transplanted have always been a concern of clinicians. This paper conducts research and analysis on this issue. STUDY DESIGN: This is a retrospective control study. All data collected was based on two IVF center between July 2011 and July 2021. We analyzed the clinical pregnancy rate and live birth rate derived from four kinds of abnormal 2PN embryos transplanted at the cleavage phases or blastocyst phases over the past 10 years. These data were statistically analyzed compared with the normal 2PN control group. RESULTS Among 50271 zygotes or embryos obtained from oocyte retrieval, 2PN, 0PN, 1PN,MNB and SERa accounted for 58.02%, 13.77%, 4.93%, 0.78% and 0.54%, respectively. After the transplantation of 3250 frozen-thawed cleavage embryos included in the study criteria, the clinical pregnancy rates of 2PN, 0PN, 1PN,MNB and SERa were: 48.6%, 18.6%, 12.4%, 25.5%, 10.7%, and the live birth rates were: 41.9%, 13.1%, 10.0%, 20.8%, 8.0%, respectively. After the transplantation of 3618 frozen-thawed blastocysts included in the study criteria, the clinical pregnancy rates of 2PN, 0PN, 1PN,MNB and SERa were: 59.4%, 48.3%, 47.6%, 51.7%, 53.1%, and the live birth rates were: 50.3%, 38.9%, 40.1%, 43.2%, 42.9%, respectively. Further analysis showed that the live birth rate of0PN, 1PN,MNB and SERacleavage embryo transplantation was only 19.1%-49.6%of normal 2PN cleavage embryo, while the live birth rate of 0PN, 1PN,MNB and SERablastocyst transplantation was 77.3%-85.9% of normal 2PN blastocyst. CONCLUSION When no normal 2PN embryos are available for transplantation, abnormal 2PN embryos are transplanted after being cultured to the blastocyst and the live birth rate close to that of a normal 2PN embryo can be obtained.