NPT100-18A reduces mitochondrial oxidative stress and rescues neuronal cell death in a human iPSC-based model of Parkinson’s disease

Abstract

Abstract Background Parkinson’s disease (PD) is a neurodegenerative disorder characterized by protein aggregates mostly consisting of misfolded alpha-synuclein (αSyn). Progressive degeneration of midbrain dopaminergic neurons (mDANs) and nigrostriatal projections result in severe motor symptoms. While the preferential loss of mDANs has not been fully understood yet, the cell type-specific vulnerability has been linked to a unique intracellular milieu, influenced by dopamine metabolism, high demand for mitochondrial activity, and increased level of oxidative stress (OS). These factors have been shown to adversely impact αSyn aggregation. Reciprocally, αSyn aggregates, in particular oligomers, can impair mitochondrial functions and exacerbate OS. Recent drug-discovery studies have identified a series of small molecules including NPT100-18A, which reduce αSyn oligomerization by preventing misfolding and dimerization. NPT100-18A and structurally similar compounds (such as NPT200-11/UCB0599, currently being assessed in clinical studies) point towards a promising new approach for disease-modification.Methods Induced pluripotent stem cell (iPSC)-derived mDANs from PD patients with a monoallelic SNCA locus duplication and unaffected controls were treated with NPT100-18A. αSyn aggregation was evaluated biochemically and reactive oxygen species (ROS) levels were assessed in living mDANs using fluorescent dyes. Adenosine triphosphate (ATP) concentrations were measured using a luminescence-based assay and neuronal cell death was evaluated by immunocytochemistry.Results Compared to controls, patient-derived mDANs exhibited increased αSyn aggregation, higher overall ROS levels, reduced ATP concentrations, and increased neuronal cell death. NPT100-18A-treatment rescued αSyn aggregation and neuronal cell death to control levels and importantly attenuated mitochondrial oxidative stress in a compartment-specific manner.Conclusions Our findings demonstrate that NPT100-18A limits αSyn aggregation and associated neurodegeneration in a human in vitro model of PD. In addition, we provide a first mechanistic insight into how a compartment-specific antioxidant effect in mitochondria might contribute to the neuroprotective effects of NPT100-18A.