Abstract
Objectives:
This in-vitro study aimed to evaluate the effect of 4 irrigation solutions at 3 different temperatures on the dentinal tubule penetration of bioceramic-based sealer after root canal obturation using the warm vertical compaction method using a laser scanning confocal microscope (CLSM).
Materials and Methods:
180 human maxillary incisors were used. After root canal preparation, different final irrigation solutions [distilled water (control), ethylenediaminetetraacetic acid (EDTA), peracetic acid, or 1-hydroxyethylidene-1,1-bisphosphonate(HEBP)] at different temperatures (25, 36, or 60˚C) were used. Obturation was performed with the warm vertical compaction using a bioceramic-based sealer mixed with a fluorophore dye. Three horizontal sections were obtained from each specimen. Samples were observed using CLSM. The maximum penetration depth, penetration area, and percentage of penetration were measured. Data were compared using the Shapiro-Wilk test and a three-way robust ANOVA with the WRS2 package (P<0.05).
Results:
The effects of different temperatures on all parameters were similar, regardless of different cross-sectional areas and solutions (P>0.05). With the increase in temperature, the maximum penetration depth values increased in the EDTA and peracetic acid groups and decreased in the HEBP group (P>0.05). Additionally, with the increase in temperature, the sealer penetration area and the total percentage of penetration increased in both the EDTA and HEBP groups (P>0.05).
Conclusions:
According to the results of this study, heating final irrigation solutions can increase the penetration of root canal sealers into dentinal tubules.
Clinical Relevance:
· Dentin tubule penetration of root canal sealer is considered clinically significant.
· Heating irrigation solutions can enhance the penetration of sealers into dentinal tubules.