Indirect Organogenesis of Platycladus Orientalis L.

Abstract

Abstract In conifer species vegetative propagation has many limitations, from low germination rates to the difficulty of rooting explants. Plant in vitro culture has long been used for multiplication, genetic improvement and conservation of elite traits. In this work it has been determined the influence of several parameters on the in vitro culture of Platycladus orientalis L.: the culture media, the type of explant, light/dark growth conditions and vernalisation period, specifically, on three different phases of development: germination, callus induction and indirect organogenesis. The best explant for callus induction was the cotyledon and the ideal medium for its culture was Murashige-Skoog under lighting conditions. In the case of indirect organogenesis, the better-differentiated explants grew up with light and LePoivre medium. In germination, the vernalisation of seeds during two months subjected to 4ºC had a positive influence. The best medium, which produced the greatest number of roots and the greatest length, was Murashige-Skoog. Also, it has determined the important role of seed coats in germination.