Acute phase protein profile of sheep post-laparoscopy

Abstract

Abstract This study aims to evaluate the acute phase response (APR) of sheep submitted to different intraabdominal pressure (IAP) during laparoscopy, with no other invasive procedure, since the ovine model is also of particular interest for studies in humans. Ten healthy nonpregnant adult Santa Ines ewes were randomly submitted to a crossover study using different IAPs: 0 mmHg (G1), 10 mmHg (G2), 12 mmHg (G3), and 15 mmHg (G4). They were anesthetized and mechanically ventilated (VT=15 ml.kg−1) with positive end-expiratory pressure (3 cmH2O) and FiO2=1.0. The targeted acute phase proteins (APP) were albumin (Alb), transferrin (Tf), apolipoprotein A1 (Apo A1), haptoglobin (Hp), ceruloplasmin (Cp), α1 acid glycoprotein (α1-AGP) and α1 antitrypsin (α1-ATT), measured before (T0), 24h (T1), 72h (T2), 7 days (T3) and 14 days (T4) after the procedure, with their respective percentage change calculated. Statistical analysis consisted of two-way ANOVA for repeated measures followed by Holm-Šídák for multiple comparisons. In G4, Alb and Tf decreased at T3 and T4. Apo A1 decreased in all groups except in G4. Hp, Cp, and α1-AGP presented a similar behavior, however, Cp had the highest percentage change at T3 and T4. In G4, α1-ATT decreased at T3 and T4, but increased in all other groups. Albumin, ceruloplasmin and haptoglobin concentrations and percentage change best characterized the dynamics of the APR in this experimental model. More obvious changes were observed with the highest IAP (15 mmHg) than in other groups at 7- and 14-days post-laparoscopy, and postoperative care longer than this is advised.