Circulating miR-182-5p for protection of endothelial function from ADMA–induced injury inelderly coronary artery

Abstract

Abstract Background To investigate the correlation between the expression level of miR-182-5p and endothelial function in elderly individuals. Methods One thousand and fifty-eight patients > 60 years old living in the Pingguoyuan area in China were enrolled consecutively and were divided into four groups according to the results of reactive hyperemia index (RHI), and asymmetric dimethylarginine (ADMA). Gruop1 (n = 294):RHI ≥ 1.67 and ADMA ≤ 0.7umol/l (named the normal endotheltial function group); Group2 (n = 244):RHI ≥ 1.67 and ADMA > 0.7umol/l; Group3 (n = 242): RHI < 1.67 and ADMA ≤ 0.7umol/l; Group4 (n = 278):RHI < 1.67 and ADMA > 0.7umol/l(named the endotheltial dysfunction group). The association between miR-182-5p level, RHI and ADMA were analyzed using univariate correlation analysis and multiple regression analysis. Results RT-PCR results showed that the expression level of miR-182-5p in the endothelial dysfunction group was significantly lower than that in the normal endothelial function group. In addition,we found that the expression level of plasma miR-182-5p was negatively correlated with plasma ADMA. Conclusion MiR-182-5p had a protective effect on endothelial function and may be a potential therapeutic target for atherosclerosis in elderly individuals.