Biosynthesis and characterization of silver nanoparticles from Punica granatum (pomegranate) peel waste and its application to inhibit foodborne pathogens-Reference-Cited by-同舟云学术

Biosynthesis and characterization of silver nanoparticles from Punica granatum (pomegranate) peel waste and its application to inhibit foodborne pathogens

Published:2023-07-12 Issue: Volume: Page:
ISSN:
Container-title:
language:
Short-container-title:

Author:

Farouk Salma M.¹,Abd-Elhalim Basma T.²,Mohamed Reham M.¹,Arabe Naira M.¹,Hussain Ahmed A. T.¹,Mostafa Mostafa E.¹,Hemdan Bahaa³,Sedeek Salwa M.⁴,Bakry Ashraf⁵,Ebeed Naglaa⁵,Elhariry Hesham⁶,Galal Ahmed⁷,Abu-Hussien Samah H.²

Affiliation:

1. New Programs, Faculty of Agriculture, Ain Shams University

2. Agricultural Microbiology Department, Faculty of Agriculture, Ain Shams University

3. National Research Center

4. Agriculture Biochemistry Department, Faculty of Agriculture, Ain Shams University

5. Genetics Department, Faculty of Agriculture, Ain Shams University

6. Food Science Department, Faculty of Agriculture, Ain Shams University

7. Poultry Production Department, Faculty of Agriculture, Ain Shams University

Abstract

Abstract Despite their role in controlling pathogens, the misuse of antibiotics leads to the antibiotic resistance phenomenon. The biosynthesis process of silver nanoparticles from plant extracts has earned wide regard worldwide because of its eco-friendly characteristics. The current study aims to biosynthesize and characterize silver nanoparticles from pomegranate peel waste PPW-AgNPs as well as test their antimicrobial potentials against foodborne pathogens. Ultraviolet (UV) spectrum, X-ray diffraction analysis (XRD), Zeta potential, Fourier transform infrared (FTIR) and scanning electron microscopy (SEM) analysis were used to characterize the produced PPW-AgNPs. The colour change from yellow to brown, the detection of a peak in a UV spectrum at 440 nm of 0.9 SPR, and four intense peaks in XRD and SEM analysis revealed the existence of spherical particles of PPW-AgNPs. Zeta potential was − 0.03 revealing the negative charge of PPW-AgNPs. Eight foodborne pathogenic strains (6 bacteria and 2 fungal strains) Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 8379, Klebsiella pneumoniae ATCC 00607, Salmonella typhi DSM 17058, Shigella sonnei DSM 5570, Aspergillus flavus ATCC 9643, and Rhizopus oryzae ATCC 96382 were used to test the inhibitory potential of PPW-AgNPs. All silver nanoparticles exhibited high antimicrobial activity against all tested strains. The most sensitive strain was B. subtilis while the highest resistant one was R. oryzae with IZD of 4.5 and 0.96cm. Pomegranate peel waste plays a key role in the simple, eco-friendly green synthesis of extracellular stable AgNPs as an alternative source for harmful chemical disinfectants.

Publisher

Research Square Platform LLC

Reference27 articles.

1. Uddin, T. M. et al. Antibiotic resistance in microbes: History, mechanisms, therapeutic strategies and future prospects. Journal of Infection and Public Health vol. 14 1750–1766 Preprint at https://doi.org/10.1016/j.jiph.2021.10.020 (2021).

2. Tagliabue, A. & Rappuoli, R. Changing priorities in vaccinology: Antibiotic resistance moving to the top. Frontiers in Immunology vol. 9 Preprint at https://doi.org/10.3389/fimmu.2018.01068 (2018).

3. Evaluating the antimicrobial resistance patterns among major bacterial pathogens isolated from clinical specimens taken from patients in mofid children’s hospital, Tehran, Iran: 2013–2018;Azimi T;Infect Drug Resist,2019

4. Murugaiyan, J. et al. Progress in Alternative Strategies to Combat Antimicrobial Resistance: Focus on Antibiotics. Antibiotics vol. 11 Preprint at https://doi.org/10.3390/antibiotics11020200 (2022).

5. Ahmad, B. et al. Phyto-fabrication, purification, characterisation, optimisation, and biological competence of nano‐silver. IET Nanobiotechnology vol. 15 1–18 Preprint at https://doi.org/10.1049/nbt2.12007 (2021).