Affiliation:
1. Department of Reproductive Medicine, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing,China
Abstract
Abstract
Aim To identify the shared gene signatures and biological mechanism in recurrent implantation failure and decreased embryo quality undergoing in vitro fertilization.Methods Relevant microarray data were selected from the GEO to screen genes that differentially expressed in response to decreased embryo quality and RIF.Results We discovered upregulated and downregulated genes. Simultaneously, we identified hub genes associated with decreased embryo quality and RIF. GO and KEGG analysis results revealed a high correlation with hypoxia-induced, inflammatory, cell-cell junction, and ubiquitin ligase complex. HIF1A, Jun, SP1, SIRT1, and POU2F1, considered TFs for decreased embryo quality and RIF.Conclusion This study explored the molecular mechanisms underlying these features and screened potential biological targets associated with decreased embryo quality and RIF.
Publisher
Research Square Platform LLC
Reference72 articles.
1. Single-cell transcriptome profiling of the human endometrium of patients with recurrent implantation failure;Lai ZZ;Theranostics,2022
2. Recurrent Implantation Failure-update overview on etiology, diagnosis, treatment and future directions;Bashiri A;Reprod Biol Endocrinol,2018
3. Selection based on morphological assessment of oocytes and embryos at different stages of preimplantation development: a review;Ebner T;Hum Reprod Update,2003
4. Baart EB, Macklon NS, Fauser BJ. Ovarian stimulation and embryo quality. Reproductive biomedicine online 2009, 18 Suppl 2, 45–50.
5. Assessment of human embryo development using morphological criteria in an era of time-lapse, algorithms and 'OMICS': is looking good still important?;Gardner DK;Mol Hum Reprod,2016