Mutations of Dipylidium caninum Cestode diagnosis of Dogs in Baghdad city, Iraq

Abstract

Abstract Objective. This study’s goal included a traditional and molecular detection of Dipylidium caninum cestode in dogs in Baghdad city. Materials and Methods. conducted 22 mature cestode obtained from 15 intestines of dead 100 dogs for morphological description by necked eye to see the D.caninum segments, body’s length and proglottids; and for conventional-PCR technique by amplified two primer pairs; DC28SrRNA and mtDNA gene, sequencing and analysis of molecular data. Results. were for the first time in dogs in Iraq and Local D.caninum Iraqi isolates with accession no. MZ677330, OL413446, OL460637 shared 99.93% identity with South Africa and 93.75% identity with Viet Nam sequence (accession no. MH045471.1 and ON248386.1 respectively). Local D.caninum Iraqi isolates with accession no. OL460638 and OL466918 shared related 97.63% identity to those from New Zealand (accession no. MG57541.1); two isolates from USA shared related 93.36% identity with accession no. AF023120.1 and MH182478.1; and Iran shared related 93.57% identity with accession no. MG774549.1) with mutations recording minor variance in genetic (0.01) across all comparison isolates. Also mtDNA gene partial sequences with accession no. OR250020.1 shared 98-99% with USA (accession no. OK523385.1 and MG587892.1) and China (accession no. OP620562.1) with substitution through sequences: deletion of OR250020.1 (9,-/C).