Probiotic properties and antioxidant activities of Lactiplantibacillus plantarum HY7720 and its fermentation characteristics in plant-based materials

Abstract

Abstract Lactiplantibacillus plantarum HY7720 was screened from 22 strains of lactic acid bacteria (LAB) that were isolated from different plant foods, and its growth ability and extracellular vitamin B12-producing capacity in vitamin B12-deficient medium were investigated. HY7720 was resistant to the conditions of the gastrointestinal tract (GIT) and showed high adhesion property to human intestinal epithelial cells. Moreover, the results showed that HY7720 recovered the gene expression levels of tight junction-associated proteins (TJPs) and inhibited the secretion levels of pro-inflammatory cytokines in tumor necrosis factor (TNF)-α-stimulated Caco-2 cells. Furthermore, the ability of HY7720 to ameliorate H2O2-induced cytotoxicity in Caco-2 cells was inhibited by mitogen-activated protein kinase (MAPK) inhibitors. This study also investigated the nutritional qualities of three plant-based materials (brown rice, white rice, and soy milk) fermented using HY7720. Collectively, HY7720 could be used as a promising probiotic strain for the prevention of nutritional deficiencies among people on vegetarian diets.