Effects of a dendritic cell vaccine loaded with whole tumor antigen in hu-PBL-SCID mice bladder cancer

Abstract

Abstract Background: The effects of dendritic cell (DC) vaccine loaded with whole tumor antigen on bladder cancer model in human peripheral blood lymphocytes (hu-PBL) -severe combined immunodeficient (SCID) mice was ivestigated. Methods: The peripheral blood mononuclear cells (PBMCs) were isolated from human peripheral blood, and were induced into DCs by co-culture with recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF) and recombinant human interleukin 4 (rhIL-4) in vitro. The DC vaccine were produced by co-culturing with whole tumor antigen which was purified through freezing and melting T24 cells. The animal model of tumor-bearing mice was established by SCID mice intraperitoneally injected with 4×107 hu-PBL and subcutaneously injected with 3×106 T24 cells. Animals were randomly allocated to an experimental group or control group. After T24 cells injected into SCID mice for 5 weeks, the mice were treated with DC vaccine or phosphate buffered saline (PBS). Human IgG level in the blood plasma of mice was detected by enzyme linked immunosorbent assay (EILSA), and human CD3+, CD4+, CD8+T cells in blood and spleen cells of mice were detected by flow cytometry (FCM) analysis. The tumor volume in each group were records. Matrix metalloproteinases-7 (MMP-7) mRNA expression in tumor tissue was detected by fluorescence quantitative reverse transcription polymerase chain reaction (FQ-RT-PCR). Results and Conclusion: Tumor volumes and MMP-7 mRNA expression in tumor tissue of DC vaccine treated mice was statistically lower than PBS group (P<0.01). DC vaccine had an anti-tumor effect on hu-PBL-SCID mice bladder cancer model.