Effects of a dendritic cell vaccine loaded with whole tumor antigen in hu-PBL-SCID mice bladder cancer

Abstract

Abstract The inhibitory effects of dendritic cell (DC) vaccine loaded with whole tumor antigen on bladder cancer model in severe combined immunodeficient (SCID) mice was ivestigated. The peripheral blood mononuclear cells (PBMCs) were obtained from human peripheral blood, and were induced into DCs by co-culture with recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF) and recombinant human interleukin 4 (rhIL-4) in vitro. DC vaccine was prepared by co-culturing with whole tumor antigen. The animal model of tumor-bearing mice was established by SCID mice intraperitoneally injected with 4×107 human peripheral blood lymphocytes (hu-PBL) and subcutaneously injected with 3×106 T24 cells. Human IgG level in the blood of mice was detected by enzyme linked immunosorbent assay (EILSA), and human CD3+, CD4+, CD8+T cells in blood and spleen cells of mice were detected by flow cytometry (FCM) analysis. Animals were randomly divided into three groups, and then received the DCs, DC vaccine or phosphate buffered saline (PBS) at the 5th and 6th week after injection of tumor cells. The weight of tumor were recorded. Fuorescence quantitative reverse transcription polymerase chain reaction (FQ-RT-PCR) was used to detect the matrix metalloproteinases-7 (MMP-7) mRNA expression in tumor tissue. The weight of tumor and MMP-7 mRNA expression in tumor tissue of mice treated with DC vaccine was significantly lower than those in PBS group (P < 0.01). DC vaccine had an anti-tumor effect on hu-PBL-SCID mice bladder cancer model.