Roseobacter Weihaiensis soli sp. nov., an actinobacterium isolated from limpet collected from Golden Beach in Weihai, China

Abstract

Abstract Strain H9T belongs to the genus Roseobacter, with positive gram stain, catalase negative, oxidase negative, pink purple, rod-shaped, amastigote, isolated from lempet collected from the Golden Beach, Weihai, Shandong Province, China. The new isolates was able to grow at 4-42 °C (optimum, 25 °C), at pH 6.5-9.0 (optimum, pH 7.0), and with 0.0-11.0% (w/v) NaCl (optimum, 3.0-5.0%). By phylogenetic analysis of the 16S rRNA gene sequence, the novel marine isolate belongs to the genus Roseobacter, the related strains Roseobacter cerasinus AI77T and Roseobacter ponti MM-7T, and the pairwise sequence showed 98.5% similarity. The summary characteristics of the main fatty acids was summed feature 8 (C18:1ω7c and/or C18:1ω6c; 81.08%). The only respiratory quinoid is Q-10. The polar lipids consisted of phosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, an unknown lipid, and a small amount of an unknown phospholipid. Based on the draft genome sequence map, the strain H9T genome length was 5,351,685 bp, and the DNA G + C content was 59.8%. The average amino acid identity (AAI) of the genome of strain H9T and closely related strains, and the average nucleotide identity (ANI) and digital DNA hybridization (dDDH) values were 63.4-76.8%, 76.2-78.8%, and 13.4-19.7%, respectively. Thus, the combination of phenotypic, chemical taxonomic, and phylogenetic data suggests that strain H9T is considered a new species in the genus Roseobacter sp. The strain H9T (= KCTC 82507T = MCCC 1K04354T) was presented in November.