Equine coronavirus infection and replication in equine intestinal enteroids-Reference-Cited by-同舟云学术

Equine coronavirus infection and replication in equine intestinal enteroids

Published:2024-07-03 Issue: Volume: Page:
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Author:

Kambayashi Yoshinori¹,Nemoto Manabu¹,Ochi Akihiro¹,Kishi Daiki¹,Ueno Takanori¹,Tsujimura Koji¹,Bannai Hiroshi¹,Kawanishi Nanako¹,Ohta Minoru¹,Suzuki Tohru²^ORCID

Affiliation:

1. Equine Research Institute

2. National Institute of Animal Health

Abstract

In this study, equine intestinal enteroids (EIEs) were generated from the duodenum, jejunum, and ileum and inoculated with equine coronavirus (ECoV) to investigate their suitability as in vitro models with which to study ECoV infection. Immunohistochemistry revealed that the EIEs were composed of various cell types expressed in vivo in the intestinal epithelium. qRT-PCR and electron microscopy showed that ECoV had infected and replicated in the EIEs. These results suggest that EIEs can be novel in vitro tools for studying the interaction between equine intestinal epithelium and ECoV. (88 words)

Publisher

Springer Science and Business Media LLC

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