Fluorescent enhancement of CNDs from Cinnamon bark with L-Arginine doping for Yeast cell Imaging

Abstract

Abstract In this study, we present an economical and efficient synthesis method for carbon nanodots (CNDs) derived from cinnamon bark wood powder, supplemented with L-arginine doping at varying ratios. Extensive structural and optical characterization was conducted through techniques such as FTIR, XRD, HRTEM, DLS, UV-Vis, and PL spectra, providing a comprehensive understanding of their properties. Quantum yields (QY) were quantified for all three samples, contributing to the assessment of their fluorescence efficiency. The synthesized CNDs were successfully applied for bioimaging of yeast cells, employing fluorescence microscopy to visualize their interaction. Remarkably, L-arginine-doped CNDs exhibited enhanced fluorescence, particularly at a higher doping ratio (1:0.50), showcasing the influence of the dopant. The non-toxic nature of these CNDs was rigorously investigated, confirming their biocompatibility. This work not only contributes to the synthesis and characterization of CNDs but also highlights their potential for diverse applications, emphasizing their structural, optical, and biological attributes. The findings underscore the versatility of CNDs derived from cinnamon bark wood powder and their potential in advancing biotechnological and imaging applications.