MIA3 promotes the degradation of GSH (glutathione) by binding to CHAC1, thereby promoting the progression of hepatocellular carcinoma

Author:

Zhou Wanbiao1,Man Jing1,Zuo Shi2,Chen Tengxiang1,Zhao Xueke2,Li Haiyang1

Affiliation:

1. Guizhou Medical University

2. The Affiliated Hospital of Guizhou Medical University

Abstract

Abstract Background and purpose: MIA3 (melanoma inhibitory active protein 3)/TANGO1 (Golgi transporter component protein) plays an important role in the initiation, development and metabolism of cancer. We aimed to explore the role and underlying molecular mechanisms of MIA3/TANGO1 in the growth and migration of hepatoma cells. Method: According to the analysis of The Cancer Genome Atlas (TCGA) database, MIA3 is expressed at higher levels in hepatocellular carcinoma (HCC) tissues than in normal tissues. MIA3 gene overexpression and gene knockout was performed via lentiviral transduction. Real-time quantitative polymerase chain reaction (qRT‒PCR), immunohistochemistry, and western blotting were used to detect mRNA and protein expression in HCC tissues and cells. The in vitro function of MIA3in HCC cells was evaluated using Cell Counting Kit-8 (CCK-8), colony formation, cell migration and invasion, and flow cytometry assays. The effect of MIA3expression changes on the growth of transplanted tumours in vivo was evaluated in nude mice. Hep-G2 cells with MIA3overexpression were subjected to RNA-seq, and the downstream target gene CHAC1 (glutathione-specific γ-glutamyl cyclotransferase 1) was selected according to the results of the volcano map of gene enrichment. The relationship between MIA3 and CHAC1 was revealed by coimmunoprecipitation and confocal microscopy. Result: MIA3 expression was upregulated in HCC organizations and HCC samples in the TCGA dataset. Knocking out MIA3 inhibited the proliferation, migration and invasion of Hep-G2 cells and promoted the apoptosis of Hep-G2 cells. Overexpression of MIA3 in Huh7 cells promoted the proliferation, migration and invasion and suppressed the apoptosis of Huh7 cells. Overexpression of MIA3promoted the growth of HCC in nude mice. Overexpression of MIA3 promoted the expression of CHAC1 and the degradation of glutathione (GSH), thereby promoting the growth and metastasis of HCC cells. Knocking out MIA3 inhibited the expression of CHAC1 and slowed the degradation of GSH, thereby inhibiting the growth and metastasis of HCC cells. Conclusion: MIA3 further promotes the growth, metastasis and invasion of hepatoma cells by binding to the CHAC1 protein and promoting GSH degradation.

Publisher

Research Square Platform LLC

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