Transcriptome analysis of Large yellow croaker (Larimichthys crocea) at different growth rates

Abstract

Abstract The unsynchronized growth of large yellow croaker (Larimichthys crocea), which affects its growth efficiency, has become one of the problems for farmers. In present study, the juvenile large yellow croaker stock was sorted by size after culturing in offshore cage for 4 months, then individuals of fast-growing group (FG) and the slow-growing group (SG) were sampled respectively. High-throughput RNA-Seq was performed to explore the physiological mechanisms that influence its growth rate. Transcriptome analysis revealed 382 differentially expressed genes (DEGs), including 145 up-regulated genes and 237 down-regulated genes, compared with SG group. GO and KEGG enrichment analysis showed that DEGs was mainly involved in signal transduction pathway and biochemical metabolic pathway. The results of RT-PCR shows that igf1, fgf19, idh1 and me1 were up-regulated in fast-growing fish, while fgf1,fgfr1 and gadd45b,g were down-regulated. Inclusion, the different growth rate of large yellow croaker is closely related to cell proliferation, metabolic rate of organism and immune regulation.These findings provide a new way to understand the molecular mechanism and regulation of the growth of large yellow croaker and enrich the understanding of growth-related genes.