Abstract
A modified immune affinity gel-column (IAGC) assay was developed for rapid detection biomarkers of heat shock protein 70 (HSP70). The new method presets antibodies in a gel for rapid detection of the target antigen flowing through it. No special instruments and cumbersome processing steps were required throughout the entire process. The whole detection was completed within 10 min and a high-throughput could be achieved. Because the detection gel is in a transparent cylinder, color changes can be observed more sensitively. The detection limit (LOD) of the newly established method can be as low as 10 µg/L, which is significantly better than the existing lateral-flow strip method. In addition, the method is suitable for outdoor use, especially in laboratories with relatively simple conditions. In addition, the new detection method can also be designed into multiple detection layers in series or multiple detection columns in parallel to further improve the detection efficiency. Therefore, the new method is a fast, simple, high-throughput, qualitative and semi-quantitative detection platform, and a new idea for rapid detection.