Affiliation:
1. BBSRC/EPSRC Synthetic Biology Research Centre
2. University of Toulouse
3. University of Nottingham
Abstract
Abstract
We engineered Clostridium acetobutylicum to continuously and stably produce advanced biofuels by integrating the pSOL1 megaplasmid into the chromosome using an advanced synthetic biology approach and further integrating sadh and hydG from Clostridium beijerinckii to convert acetone to isopropanol. In a membrane bioreactor, continuously fed with a glucose mineral medium, the final strain stably produced advanced biofuels at high yield (0.31 g·g-1), titre (15.4 g·L-1) and productivities (15.5 g·L-1·h-1).
Publisher
Research Square Platform LLC