Molecular characterization of stable QTL and putative candidate genes for grain zinc and iron concentrations in two related wheat populations

Abstract

Abstract Micronutrient malnutrition is one of the most common public health problems in the world. Biofortification the most attractive and sustainable solution to surmount malnutrition requires the development of micronutrient enriched new crop cultivars. In this study, two RIL populations, ZM175/XY60 and ZM175/LX987, were used to identify QTL for grain zinc concentration (GZnC), grain iron concentration (GFeC) and thousand grain weight (TGW). Eight QTL for GZnC, six QTL for GFeC and five QTL for TGW were detected. Three QTL on chromosome arms 2DL and 4BS and chromosome 6A showed pleiotropic effects on all three traits. The 4BS and 6A QTL also increased plant height and might be Rht-B1a and Rht25a, respectively. The 2DL locus within a suppressed recombination region was identified in both RIL populations and the favorable allele simultaneously increasing GZnC, GFeC and TGW was contributed by XY60 and LX987. A QTL on chromosome arm 6DL associated only with GZnC was detected in ZM175/XY60 and was validated in a JD8/AK58 RILs using KASP marker K_AX-110119937. Both the 2DL and 6DL QTL were new loci for GZnC. Based on gene annotations, sequence variations and expression profiles, phytic acid biosynthesis gene TaIPK1-2D and nicotianamine synthase gene TaNAS10-6D were predicted as candidate genes. Their gene-based KASP markers were developed and were validated in a cultivar panel of 343 wheat accessions. This study investigated the genetic basis of GZnC and GFeC, and provided valuable candidate genes and markers for breeding Zn- and Fe-enriched wheat.