An efficient and reliable protocol on in vitro propagation of ‘Colt’ (Prunus avium F 299/2 x Prunus pseudocerasus Lind) rootstock of cherry-Reference-Cited by-同舟云学术

An efficient and reliable protocol on in vitro propagation of ‘Colt’ (Prunus avium F 299/2 x Prunus pseudocerasus Lind) rootstock of cherry

Published:2023-08-18 Issue: Volume: Page:
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Author:

Mir M. A.¹,Manzoor Ikra²,Kashani Syed Zainab³,Attia Houneida⁴,Bhat Momin Showkat⁵,Abidi Ishfaq. A.⁶,Alamer Khalid H.⁷,Dar Showket Ahmad⁸,Lone Showkat A⁹,Iesa Mohamed A. M.¹⁰,Al-Qarni Maha¹¹,Fekry Waleed M.E.¹²,Sultan Rania Mohammad Sabri¹³,Ainusairi Ghalia S.H.¹⁴,Zoubi Omar Mahmoud Al¹⁵,Alharbi Basmah M.¹⁶,Moqbel Fahmi S¹⁷,Youssef Rawa M¹⁸

Affiliation:

1. Ambri Apple Research Centre, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Pahnoo,Shopian-192303;

2. Division of Fruit Science, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir,Shalimar,Srinagar-190025;

3. Division of Vegetable Science, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir,Shalimar,Srinagar-190025;

4. Department of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia

5. Division of Floriculture and Landscape Architecture, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir,Shalimar,Srinagar-190025

6. Head KVK-Ganderbal SKUAST-K-Kashmir

7. Biological Science Department Faculty of Science and Arts, King Abdulaziz University, Rabigh 21911, Saudi Arabia.

8. Faculty of Forestry, Division of Social and Basic Science (Entomology); Watlar Ganderabl-SKUAST-K, Kashmir India

9. Department of Basic Sciences, College of Science and Theoretical Studies, Saudi Electronic University, (Jeddah-M), Riyadh, 11673, Saudi Arabia

10. Department of Physiology, Al Qunfudah Medical College, Umm Al Qura University, Mecca, Saudi Arabia

11. Department of Biology, Faculty of Sciences, University of Bisha, P.O. Box 551, Bisha 61922, Saudi Arabia

12. Department of Plant protection, Arid Land Cultivation Research Institute, City Scientific Research and Technological Applications, New Borg El-Arab City 21934 Alexandria Egypt

13. King Abdul-Aziz University Faculty of Science, Department of Biological Sciences, Saudi Arabia

14. Department of Biology college of Science Jouf University Sakaka 2014, Saudi Arabia

15. Biology Department Faculty of Science, YanbuTaibah University Yanbu El-Bahr 46423Saudi Arabia.

16. Biology Department Faculty of Science, University of Tabuk, Tabuk 71491, Saudi Arabia

17. Department of Biology, Faculty of Applied Science, Thamar University, Yemen,

18. General Commission of Agricultural Scientific Research – Damascus

Abstract

Abstract Background The 'Colt' is a triploid hybrid of P. avium and P. pseudocerasusL. grown as a cherry rootstock because of its resistance to cherry stem pitting (a debilitating virus disease), Phytophthora root rot, bacterial canker, and gopher damage. ResultsThe current study was carried out using two types of explants: forced (F1) and unforced (F2) and five types of sterilants were used to sterilize explants: 10% sodium hypochlorite (NaOCl) for 10 minutes (S1), 0.1% mercuric chloride (HgCl2) for 5 minutes (S2), ethyl alcohol for 10 seconds (S3), (S1) + (S3) & (S2) + (S3) for sterilization. During culture establishment, six growth regulator regimes viz., BA (Benzyl adenine) (0.20), (0.40) & (0.60) and BA+IBA (Indole-3-butyric acid) (0.20+0.01), (0.40+0.01), and (0.60+0.01) mg/l, two media i.e., MS (Murashige and Skoog, 1962)& WPM (Woody Plant Medium)& two types of explants viz., forced (F1) & unforced shoot tips(F2) were utilized. During shoot proliferation, MS & WPM culture medium with growth regulators viz., BA (0.20), (0.40) & (0.60), BA+IBA (0.20+0.01), (0.40+0.01) and (0.60+0.01) mg/l were used respectively. During rhizogenesis using different media (MS & WPM) with various levels of IBA (0.50), (1.00) (1.50), and (2.00) mg/l, respectively were used. The superior rooted plants were hardened in sand & cocopeat respectively. Conclusion In this experiment, maximum culture asepsis (%) obtained in unforced shoot tips(F2) with (S5),maximum explant survival (%) in forced shoot tips (F1) with (S4), and highest establishment (%) with MS medium, BA (0.6) mg/l & in forced shoot tips (F1). Significantly higher shoot proliferation is obtained with MS medium with BA (0.6) mg/l , maximum shoot number & length in WPM medium with BA (0.2) mg/l. A significantly higher rooting percentage was obtained in MS medium with IBA (1.00) mg/l with maximum ex vitro survival (%) in cocopeat.

Publisher

Research Square Platform LLC

Reference81 articles.

1. 11Department of Biology., Faculty of Sciences, University of Bisha, P.O. Box 551, Bisha 61922, Saudi Arabia.

2. 12Department of Plant protection, Arid Land Cultivation Research Institute., City Scientific Research and Technological Applications, New Borg El-Arab City 21934 Alexandria Egypt.

3. 13King Abdul-Aziz University Faculty of Science., Department of Biological Sciences, Saudi Arabia.

4. 14Biology Department Faculty of Science., University of Tabuk, Tabuk 71491, Saudi Arabia.

5. 15Department of Biology., Faculty of Applied Science, Thamar University, Yemen.