Affiliation:
1. National Research Centre
Abstract
Abstract
Sarcocystis spp. infects water buffaloes (Bubalus bubalis) causing sarcocystosis. In the present study, Sarcocystis fusiformis was recognized in Egyptian water buffaloes based on histological observation and molecular analysis of internal transcribed spacer 1( ITS1), 18S ribosomal RNA (18S rRNA) and cytochrome c oxidase subunit I (COX-1) gene fragments. Chemotherapy and vaccines against Sarcocystis spp. could potentially target proteases because they may play a crucial role in the infection. Cysteine proteases are multifunctional enzymes involved in vital metabolic processes. However, the involvement of proteases in S. fusiform infection has not yet been characterized. Here, the purification and study on some biochemical properties of protease isolated from cysts of S. fusiform were carried out. Protease with a molecular weight of 100 kDa was purified. The purified protease exhibited maximum activity at pH 6 and a temperature of 50°C. The Michaelis-Menten constant (Km), the maximum velocity (Vmax) and the turnover number (Kcat) were determined. The complete inhibition effect of cysteine inhibitors indicated that the purified enzyme is cysteine protease. The results suggested that S. fusiform proteolytic enzyme may be necessary for parasite survival in the water buffaloes by digesting host tissues. Therefore, cysteine protease could be a suitable target for vaccinations.
Publisher
Research Square Platform LLC
Reference60 articles.
1. Human infections with Sarcocystis species;Fayer R;Clinical microbiology reviews,2015
2. Verma SK, Lindsay DS, Grigg ME, Dubey JP. Isolation, Culture and Cryopreservation of Sarcocystis species. Current protocols in microbiology, 45:20d.21.21-20d.21.27 (2017).
3. Survey of Sarcocystis infection in slaughtered sheep and buffaloes in Ardabil, Iran;Daryani A;Journal of Animal and Veterinary Advances,2006
4. Sarcocystis infection in beef and industrial raw beef burgers from butcheries and retail stores: A molecular microscopic study;Ayazian Mavi S;Heliyon,2020
5. Sarcocystis spp. in human infections;Fayer R;Clinical microbiology reviews,2004