The effect of combined training and resveratrol supplementation on serum levels of adropin, vascular endothelial growth factor, nitric oxide, and gene expression of fibroblast growth factor-2 and angiostatin in myocardial infarction rats

Abstract

Abstract Objectives: The aim of current study was evaluating the effect of exercise training and resveratrol supplementation on serum levels of adropin, vascular endothelial growth factor (VEGF), nitric oxide (NO), and gene expression of angiostatin and fibroblast growth factor-2 (FGF-2) in myocardial infarction rats Methods: 40 male Wistar rats four weeks after cardiac infarction were randomly divided into four groups: 1: control group (C-MI, n=10) 2: resveratrol group (Res-MI, n=10) 3: exercise group (Ex+MI, n=10) and 4: exercise group with resveratrol supplement (Ex+Res, n=10) were divided. The Ex+MI group and the Ex+Res group performed running on a treadmill for 10 weeks (5 sessions per week and each session lasting 50 minutes at a speed of 17 meters per minute). The serum levels of adropin, VEGF and NO were measured by ELISA method and expression of angiostatin and FGF-2 genes were measured by Real Time-PCR. The statistical analysis of the obtained data was done using the one-way analysis of variance test. Results: The findings of the present study showed that the level of NO, VEGF and adropin in EX+Res, Ex+MI and Res+Mi groups were significantly different from C-MI. Also, the difference between Ex+Res and other groups was significant. In terms of gene expression, the highest expression of FGF-2 was in the Ex+Res group and then in the Ex+MI group, and there was a significant difference between the level of Ex+Res and the Res-MI group. Also, the lowest angiotensin gene expression was in the Ex+Res and Ex+MI groups, which showed a significant difference compared to the other two groups. Conclusion: Ten weeks of aerobic exercise training and resveratrol supplementation lead to increase the levels of VEGF, adropin, NO as well as the mRNA expression of FGF-2 and decrease the levels of angiostatin in MI rat’s model.