Histopathological changes following immunization with recombinant leukemia inhibitory factor

Abstract

Abstract Background Leukemia inhibitory factor (LIF) is a multifunctional glycoprotein of the interleukin-6 superfamily that regulates many physiological processes as well as pathological conditions. LIF plays an important role in the initiation and progression of solid tumors as well as its role in leukemia suppression. The purpose of this study is to determine whether exogenous LIF has any side effects in liver and kidney tissues that have receptors for this cytokine in immunized mice compared with controls.Methods LIF and complete Freund's adjuvant were injected subcutaneously for the test group in the initial injection. Freund's incomplete adjuvant was injected intraperitoneal for the last injection. Phosphate-buffered saline was used with the adjuvant for the control group.Results The liver structure in the control group was normal. However, the LIF immunized group exhibited irregular sinusoidal plates and hyperemia in central and portal veins compared to the control group. The kidney tissue in the control group was also normal, with preserved tubule shapes and no cell destruction or degeneration. In the LIF injected group, hyperemia and an increase in the urinary space were observed, but the overall kidney tissue condition was similar to the control group, with no tissue destruction or infiltration of mononuclear cells, and the renal corpuscle and glomerular coil remained normal.Conclusion The results indicated that while the injection of exogenous LIF into the mouse body caused changes and destructive effects on the liver tissue, including the development of an inflammatory condition in the liver, it had no discernible effect on the kidney tissue.