Affiliation:
1. Zhuhai International Travel Healthcare Center
2. Futian district center of disease conctrol and prevention
Abstract
Abstract
In the current study, an one pot quadruplex RT-qPCR assay was established and evaluated. The assay’s limit of detection could reach as low as 101 copies/reaction, with good repeatability profile and no cross-reaction with other respiratory pathogens. During clinical evaluation both by blinded samples and real clinical samples, the assay exhibited a 100% coincidence rate with individual commercial RT-qPCR assays. To the best of our knowledge, this is the first report on the simultaneous subtyping influenza A virus into H1, H3, N1, and N2 by one pot quadruplex RT-qPCR assay, which could improve the preparedness for future influenza outbreaks.
Publisher
Research Square Platform LLC
Reference19 articles.
1. Morbidity and mortality of the Russian population from acute respiratory viral infections, pneumonia and vaccination;Bilichenko TN;Ter Arkh,2018
2. Viral aetiology of influenza-like illnesses and severe acute respiratory illnesses in Morocco, September 2014 to December 2016;Bimouhen A;J global health,2022
3. Simultaneous detection of influenza A subtypes of H3N2 virus, pandemic (H1N1) 2009 virus and reassortant avian H7N9 virus in humans by multiplex one-step real-time RT-PCR assay;Cui D;SpringerPlus,2016
4. Real-time RT-PCR assays for type and subtype detection of influenza A and B viruses;Daum LT;Influenza Other Respir Viruses,2007
5. Rapid multiplex reverse transcription-PCR typing of influenza A and B virus, and subtyping of influenza A virus into H1, 2, 3, 5, 7, 9, N1 (human), N1 (animal), N2, and N7, including typing of novel swine origin influenza A (H1N1) virus, during the 2009 outbreak in Milwaukee, Wisconsin;He J;J Clin Microbiol,2009