1. Supporting information
2. Supplementary Fig. 1. The antibody used in the syndecan-1 ELISA kit wasãconfirmed to cross-react with heparin. (A) Concentrations of syndecan-1 determined using an ELISA kit and serum extracted from mice treated with saline (n = 6) or heparin (n = 7) at five minutes before the administration of anti-MHC class I antibody (clone 34-1-2s). No significant difference was observed. (B) Concentrations of syndecan-1 determined using an ELISA kit and serum extracted from mice treated with saline (n = 1) or heparin (n = 1) and in solutions of each concentration of heparin. We confirmed that heparin itself increases the syndecan-1 concentration. NS, not significant
3. Supplementary Fig. 2. The anti-MHC class antibody did not affect the expression of the Hpse2 gene in peripheral macrophages and neutrophils. (A) 34-1-2s Antibody or control IgG (4.5 mg/g body weight) was administered intravenously to mice. Thirty minutes after administration, the macrophages were isolated, and the expression of Hpse2 mRNA was determined using real-time RT-PCR with normalization according to the expression of Actb. (B) Neutrophils isolated from mouse peripheral blood were incubated for 30 minutes with anti-MHC class I antibody or control IgG (0.5 mg/mL). The expression of Hpse2 mRNA was determined as described above. (A,B) Data are expressed as the mean ± SD. NS, not significant
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