SARS-CoV and SARS-CoV-2 display limited neuronal infection and lack the ability to transmit within synaptically connected axons in stem cell-derived human neurons

Author:

Luczo Jasmina M.1,Edwards Sarah J.2,Ardipradja Katie1,Suen Willy W.1,Au Gough2,Marsh Glenn A.2,Godde Nathan1,Rootes Christina L.2,Bingham John1,Sundaramoorthy Vinod1

Affiliation:

1. Australian Centre for Disease Preparedness, Commonwealth Scientific and Industrial Organisation

2. Commonwealth Scientific and Industrial Organisation

Abstract

Abstract Sarbecoviruses such as SARS and SARS-CoV-2 have been responsible for two major outbreaks in humans, the latter resulting in a global pandemic. While sarbecoviruses primarily cause an acute respiratory infection, they have been shown to infect the nervous system. However, mechanisms of sarbecovirus neuroinvasion and neuropathogenesis remain unclear. In this study, we examined the infectivity and trans-synaptic transmission potential of the sarbecoviruses SARS and SARS-CoV-2 in human stem cell-derived neural model systems. We demonstrated limited ability of sarbecoviruses to infect and replicate in human stem cell-derived neurons. Furthermore, we demonstrated an inability of sarbecoviruses to transmit between synaptically connected human stem cell-derived neurons. Finally, we determined an absence of SARS-CoV-2 infection in olfactory neurons in experimentally infected ferrets. Collectively, this study indicates that sarbecoviruses exhibit low potential to infect human stem cell-derived neurons, lack an ability to infect ferret olfactory neurons, and lack an inbuilt molecular mechanism to utilise retrograde axonal trafficking and trans-synaptic transmission to spread within the human nervous system.

Publisher

Research Square Platform LLC

Reference65 articles.

1. Neurology and neuropsychiatry of COVID-19: a systematic review and meta-analysis of the early literature reveals frequent CNS manifestations and key emerging narratives;Rogers JP;J Neurol Neurosurg Psychiatry,2021

2. Neurologic Manifestations in a Prospective Unselected Series of Hospitalized Patients With COVID-19;Garcia-Monco JC;Neurol Clin Pract,2021

3. Frequency of Neurologic Manifestations in COVID-19: A Systematic Review and Meta-analysis;Misra S;Neurology,2021

4. Neurologic manifestations in hospitalized patients with COVID-19: The ALBACOVID registry;Romero-Sanchez CM;Neurology,2020

5. Neurologic manifestations in hospitalized patients with COVID-19 in Mexico City;Flores-Silva FD;PLoS ONE,2021

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3