Analysis of Antimicrobial resistance gene in Staphylococcus aureus by Gene Networking using Cytoscape

Abstract

Abstract Antibiotic resistance One of the major global issues of this century is AMR among microorganisms, which is still a challenge for mankind. It's crucial to understand the genes responsible for AMR and how those genes create resistance in order to solve this issue effectively. Staphylococcus aureus, which has AMR genes imparting resistance against numerous antibiotics, was the main subject of our investigation. We performed a phylogenetic study to examine the gene network's evolutionary history. To increase our understanding, a gene interaction network with 46 functional partners was built from String Database and Cytoscape and examined. Clustering analysis revealed one cluster (C1) that was linked to a multidrug resistance mechanism. According to Cluego's enrichment analysis, 20 genes are significantly involved in biological processes, as are 14 genes in cellular components and 16 genes in molecular functions. RpoB, RpoC, FusA, RplI, and RpsL had the most interactions by Cytohubba when the degree and closeness of the network were studied, according to the analysis of the gene interaction network. Understanding the molecular basis of AMR requires analysis of the enriched pathways and Gene Ontologies (GO). The suggested study may also help researchers find new ways to battle the multi-drug resistance of Staphylococcus aureus.