How to enhance the effects of Platelet Rich Plasma on tissue regeneration: AMPLEX PLUS technology, a new strategy.

Abstract

Abstract The employment of Platelet-Rich Plasma (PRP) has generated promising results in several fields of aesthetic and regenerative medicine. However, PRP research represents a continuing expanding field, due in part to the lack of standardized protocols and consistent clinical trials. A significant progress has been achieved with the analysis and the elucidation of mechanisms of exosomes. They are extracellular vesicles surrounded by membrane with a crucial role of carriers of different substances involved in tissue repair. These bioactive molecules are growth factors and cytokines capable of stimulating cell proliferation, ensuring tissue regeneration in response to injury. Therefore, scientific research has focused on identifying other matrices rich in growth factors, cytokines, and exosomes to improve the efficacy of PRP. In the present study, we evaluated by enzyme linked immunosorbent assay (ELISA) the concentration trends of 20 growth factors and cytokines in plasma, PRP and PRP with a new technology, a compound derived from colostrum enriched with exosomes. The results showed that the concentration of all analyzed compounds increased significantly in PRP samples with the new technology compared with samples containing only plasma or PRP, suggesting how this new strategy can improve PRP performance and make potential progress in regenerative medicine.