Affiliation:
1. Department of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technologies, Institute of Natural and Applied Sciences, Nigde Omer Halisdemir University, 51240, Türkiye
Abstract
Abstract
Antarctica vascular species are of vital importance for plant breeding and ecosystem studies due to their extreme environmental adaptabilities. However, limited molecular studies exist especially comparing Antarctica vascular species and other cereal species from the Poaceae family. In this study, the two vascular plant species; Deschampsia antarctica and Colobanthus quitensis found in the Antarctic ecosystem were molecularly characterized using 45 inter primer binding site (iPBS) retrotransposon markers. The Antarctica species were sampled from various locations in the Antarctica region during a scientific expedition. Five temperate cereal species; Avena sativa, Secale cereale, Hordeum vulgare, Triticum durum, and Triticum aestivum, and one tropical cereal species, Sorghum bicolor were also included for comparative analysis. A total of 23 individuals in two replicates all from the Poaceae family were assessed in this study. The iPBS markers produced 1506 scorable reproducible bands of which 1226 bands were polymorphic. The dendrogram based on the UPGMA clustering and principal coordinate analysis (PCoA) classified the speices into three distinct clusters. High variabilities existed between the Arctic, temperate and tropical species indicating their geographical diversity and adaptations. The results of the present study provides basis for further research on these species to identify sources of variation for climate resilience and resistance breeding for sustainable agriculture. Further study is recommended to assess the phylogenetic relationship among these species.
Publisher
Research Square Platform LLC