Assessment of Arsenic (As)-Induced Hepato-Nephro alterations, enzymatic inhibition and Neurobehavioral instability in Charles foster rats

Abstract

Abstract The extremely hazardous trace metal arsenic is ubiquitous in the Earth’s crust contaminating groundwater globally. The present study was conducted to evaluate the arsenic-induced hepato-nephro alterations, enzymatic inhibition and neurobehavioral instability and in Charles foster rats. Rats were divided into four groups (n = 6; each) weighing with 160 ± 20 g. The group I (control) received arsenic free distilled water and remaining three treatment group (group II − 5 mg kg− 1 body weight, group III; 8mg kg− 1 body weight and group IV; 12mg kg− 1 body weight) were treated with Sodium arsenite (NaAsO2) in combination with distilled water for 90 days. This experimental exposure reveals severe haematological, biochemical and histopathological changes along with neuro-behavioural disorders. The presence of arsenic in blood were analysed through Graphite Furnace Atomic Absorption Spectrophotometer (GF-AAS). Compare to control group the analysed level of arsenic is too high in blood. The saviour significantly (P < 0.0001) changes were observed in haematological and hepato-nephro biochemical parameters. In observational view the behavioural infirmities, weight loss, hair loss, keratosis and the limb paralysis seen. The study concludes that regular exposure of arsenic significantly alters the levels of antioxidants (ROS) and leads to severe cellular alteration in hepato-nephro region compare to control group.