Characterization of Exosomes Isolated from Non-Small Lung Cancer Cell (NC1975) using Improved Morphological and Surface Protein Biomarkers methods

Abstract

Abstract Lung cancer is considered one of the leading causes of death worldwide. Usually, disease detection is difficult in the early stages; new methods for diagnosis using non-invasive ways are developed using Nano-molecules called exosomes. Exosomes are cell-derived vesicles carrying different molecules (proteins, DNA, RNA, microRNA) and displaying many proteins on their membrane surfaces. In this study, NCI 1975 Cells line has been used as a source of exosomes. First, the isolation processes were carried out using different ultra-centrifugation devices. Then isolated exosomes were characterized using surface protein biomarkers. Finally, the Exosome isolated products were screened under Transmission Electron Microscopy (TEM) and compared with Scanning Electron Microscopy (SEM). The results showed that the size distributions of apoptotic vesicles (APV) 450nm and necrotic bodies (NCB) 280 nm (extracellular vesicles )were significantly different from exosomes(P < 0.001). This study confirmed the accessible use of exosomes in the diagnosis and prognosis of Non-Small Cell Lung Cancer (NSCLC).