Affiliation:
1. Indira Gandhi Krishi Vishwavidyalaya
2. International Rice Research Institute
Abstract
Abstract
Filamentous fungi Trichoderma are well known for its opportunistic lifestyle that displays a remarkable range of interactions with plants, ability to antagonize plant-pathogenic fungi, stimulate plant growth and defence responses. Gamma-ray induced genetic diversity which also results in mutant progenies and therefore forms the basis for strain improvement for traits imparting antagonistic effect as well as plant growth promoting activity. One hundred and seventy putative variants were derived from Gamma-ray irradiation of wildtype Trichoderma atroviride (T-14). Putative T. atroviride (T-14) derived variants displayed variation in cultural characteristics and through biochemical screens we were able to identify mutants which were superior siderophore and chitinase producers, phosphate solubilizers, and with improved ACC deaminase activities. Identification of superior T. atroviride (T-14) derived putative variants with chitinases, phosphatases, cellulases, ACCd, siderophore producing ability suggest the possibilities of strain improvement of T. atroviride (T-14) through gamma irradiation. Identified superior putative variants of (T-14) were also able to significantly stimulate the plant growth and can be exploited as for commercial production useful for farmers.
Publisher
Research Square Platform LLC