Genome-wide identification and expression analysis of the BAHD gene family in Leonurus japonicus-Reference-Cited by-同舟云学术

Genome-wide identification and expression analysis of the BAHD gene family in Leonurus japonicus

Published:2024-05-15 Issue: Volume: Page:
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Author:

Wang Qing¹,Zhang Jiaxin¹,Lv Wenhan¹,Guo Tongtong¹,Yi Yuxiao¹,Yang Fengtang¹,Xu Jianing¹

Affiliation:

1. Shandong University of Technology

Abstract

Background Acylation represents a pivotal biochemical process that is instrumental in the modification of secondary metabolites throughout the growth and developmental stages of plants. The BAHD acyltransferase family within the plant kingdom predominantly utilizes coenzyme A thioester as the acyl donor, while employing alcohol or amine compounds as the acceptor substrates to facilitate acylation reactions. These reactions are crucial for the biosynthesis of a diverse array of products, including but not limited to lignin monomers, anthocyanins, terpenoids, and alkaloids. Despite the importance of the BAHD acyltransferases in the context of plant biochemistry, an in-depth investigation into the BAHD gene family within the species Leonurus japonicus (L. japonicus) has yet to be conducted. Results Using bioinformatics approaches, the LjBAHD gene family members in the genome of L. japonicus were identified and characterized including gene structure, conserved motifs, cis-acting elements, and potential gene functions. In total, we detected 47 LjBAHD genes, unevenly distributed across 10 chromosomes of L. japonicus, with subcellular localization predictions indicating a predominant cytoplasmic localization. Phylogenetic analysis grouped the LjBAHD genes into four distinct clades. Cis-acting element analysis revealed that the expression of BAHD genes is predominantly regulated by light and hormonal signals. Examination of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways demonstrated remarkably enrichment of LjBAHD genes in the biosynthetic pathways for Stilbenoids, diarylheptanoids and gingerol biosynthesis (Ko00945), as well as Flavonoid biosynthesis (Ko00941) and Phenylpropanoid biosynthesis (Ko00940). To elucidate the roles of BAHD genes in various tissues of L. japonicus, the expression profiles of LjBAHD family members across different organs were scrutinized. Notably, a detailed study was conducted on a specific HCT gene (i.e. LjBAHD25) within the BAHD gene family. Analysis of its expression patterns suggested a role for LjBAHD25 in the phenylpropanoid metabolism pathway in L. japonicus, contributing to the biosynthesis of secondary metabolites with unique bioactivity. Conclusion The findings of this study have established a scientific foundation for the subsequent development and functional validation of the BAHD gene family in L. japonicus.

Publisher

Springer Science and Business Media LLC

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