Phenotypic and molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) circulating into environments in Ouagadougou, Burkina Faso.-Reference-Cited by-同舟云学术

Phenotypic and molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) circulating into environments in Ouagadougou, Burkina Faso.

Published:2024-05-24 Issue: Volume: Page:
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Author:

Ouédraogo Ganamé Abasse¹,Kaboré Boukaré¹,Traoré Roukiatou¹,Ouédraogo Henri Sidabéwindin¹,Cissé Hama¹,Bassolé Ismael Henri Nestor²,Tchoumbougnang François³,Savadogo Aly¹

Affiliation:

1. Laboratoire de Biochimie et Immunologie Appliquées (LaBIA) au Burkina Faso, Université Joseph KI-ZERBO

2. Laboratoire de biologie moléculaire, d'épidémiologie et de surveillance des bactéries et virus transmis par les aliments (LaBESTA) au Burkina Faso, Université Joseph Ki-ZERBO

3. Institut des Sciences Halieutiques (ISH) à Yabassi au Cameroun, Université de Douala

Abstract

Meticillin-resistant Staphylococcus aureus (MRSA) diagnostic techniques are not very easy in rattan practices in Burkina Faso and West African. This is main difficulty of good MRSA surveillance in this region. Thus, to consolidate MRSA data in locality that, 33 strains of S. aureus were tested for susceptibility to cefoxitin according using standard method. In addition, the genetic determinants including mecA gene and SCCmec cassettes were searched using conventional PCR technic to same strains. These protocols made it possible to obtain prevalence’s of 42.42% (14/33) and 18.18% (6/33) respectively of S. aureus resistant to cefoxitin and positive in Chrom MRSA ID® agar medium. From 33 S. aureus studied, 6 strains (18.18%) and 7 strains (21.21%) all of human origin were respectively positive for mecA resistance gene and SCCmec IVa cassette. This study brought circulating MRSA in the city of Ouagadougou. These MRSA harbor mecA resistance genes and SCCmec IVa cassettes.

Publisher

Research Square Platform LLC

Reference30 articles.

1. Classification of Staphylococcal Cassette Chromosome Mec (SCC Mec): Guidelines for Reporting Novel SCC Mec Elements;(IWG-SCC), International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements;Antimicrobial Agents and Chemotherapy,2009

2. Evaluation for the Novel MecC Methicillin Resistance among Methicillin Resistant Staphylococcal Isolates in Two Egyptian University Hospitals;Rania AA;Archives of Clinical Microbiology,2018

3. Abasse, Ouédraogo Ganamé, Kaboré Boukaré, Emmanuel Sampo, Rober Bouda, Hama CISSE, Kpoda Stéphane, Ismail Odetokun, Adama Sawadogo, Bassolé Henri Nestor, and Aly SAVADOGO. 2021. “Spread and Antibiotic Resistance Profile of Pathogens Isolated from Human and Hospital Wastewater in Ouagadougou.” Microbes and Infectious Diseases 0 (Article In Press): 1–14. https://doi.org/10.21608/mid.2021.72261.1143.

4. Abimana, Justus B., Charles D. Kato, and Joel Bazira. 2019. “Methicillin-Resistant Staphylococcus Aureus Nasal Colonization among Healthcare Workers at Kampala International University Teaching Hospital, Southwestern Uganda.” Canadian Journal of Infectious Diseases and Medical Microbiology 2019 (ID 4157869): 8. https://doi.org/10.1155/2019/4157869.

5. Accarias, Solène. 2014. “Impact Du Phénotype Des Macrophages Résidents Sur La Nature de La Réponse Inflammatoire Précoce Lors d’une Infection Par Staphylococcus Aureus.” Université Paul Sabatier Toulouse III. https://scholar.google.com/scholar?hl=fr&as_sdt=0%252C5&q=Accarias+S.+“Impact+du+phénotype+des+macrophages+résidents+sur+la+nature+de+la+réponse+inflammatoire+précoce+lors+d’une+infection+par+Staphylococcus+aureus%252C”+Université+Paul+Sabatier+Toulouse+III%252.