Raltitrexed combined with carbon nanoparticles for lymphatic chemotherapy: on the in vitro cytotoxic behavior of HCT-116 cells

Abstract

Abstract Objective Carbon nanoparticle has a lymphatic tracing effect, which can be combined with anti-tumor drugs to induce lymph nodes and have a strong inhibitory effect on tumor cells. Evaluate the feasibility and effectiveness of the method. Methods： CCK8 method was used to detect 1. IC50% (50% minimum lethal concentration) of CNP (50ug/ml-1500ug/ml) and RTX (10ug/ml-100ug/ml) on colorectal cancer cells HCT116, 2. Western blotting experiment (western blot, WB) to detect the effect of CNP (500ug/ml) on the apoptosis pathway of colorectal cancer cells HCT116. 3. The inhibitory effect of CNP+RTX (500ug/ml+40ug/ml) on intestinal cancer cells HCT116. Compare the inhibitory effect of pure RTX (40ug/ml). Results: 1. The carbon nanoparticles had antiproliferative toxicity to HCT-116 cells in vitro.2. The IC50% of CNP and RTX on intestinal cancer cells HCT116 were 500ug/ml and 40ug/ml, respectively.3. Carbon nanoparticles promote the apoptosis of intestinal cancer cells HCT116.4. In the in vitro cytotoxicity test, CNP combined with RTX has a strong inhibitory effect on intestinal cancer cell HCT116, which is superior to RTX alone. Conclusions: In vivo studies suggest that colorectal cancer cells HCT116 have obvious apoptosis under the treatment of carbon nanoparticles, and CNP+RTX has a significant inhibitory effect on colorectal cancer cells HCT116. Carbon nanoparticles raltitrexed combined with carbon nanoparticles may be a potential delivery system during lymphatic chemotherapy.