Abstract
This study investigated watermelon diseases characterized by contrasting climatic conditions in the Sylhet and Natore Districts of Bangladesh. Sylhet experiences lower temperatures and high rainfall, while Natore has higher temperatures and low rainfall. In these survey regions, 40 watermelon fields were selected, and 10 diseases were observed, including 4 fungal, 3 bacterial, 2 water mold, and 1 viral disease. The observed diseases were anthracnose, Cercospora leaf spot, Fusarium wilt, Gummy stem blight, Downy mildew, Phytophthora fruit rot, Bacterial fruit blotch, Angular leaf spot, Yellow vine, and Watermelon mosaic disease. Molecular analysis was performed in the Plant Pathology Laboratory at Sher-e-Bangla Agricultural University using specific primers for the fungal (ITS1/ITS4) and bacterial (27F/1492R) DNA regions, and nine pathogen species were identified, excluding the causal organism of the viral disease. The identified pathogens included Colletotrichum orbiculare, Cercospora citrullina, Fusarium oxysporum, Stagonosporopsis cucurbitacearum, Pseudoperonospora cubensis, Phytophthora capsici, Acidovorax citrulli, Pseudomonas syringae, and Serratia marcescens. The sequencing of the identified pathogens revealed high homology (98.91–99.71%) with known sequences in the GenBank database. Phylogenetic analysis revealed six clusters for fungal and water mold pathogen isolates and three for bacterial isolates, where the percentages of replicate trees were 100% in all the cases. Among the identified diseases, the highest disease occurrence was caused by Fusarium wilt (47.5%), followed by Gummy stem blight (41.5%) in the Sylhet region and by angular leaf spot (37.5%), followed by yellow vine (33%) in the Natore area. Fusarium wilt also has a high disease intensity, demonstrating its devastating impact on yield. This study highlights the influence of environmental conditions on disease incidence and underscores the need for tailored management strategies. These findings provide a foundation for developing targeted disease management practices for sustainable watermelon cultivation in Bangladesh.