Abstract
Scrophularia umbrosa is a medicinal plant used as a traditional herb. This plant is one of the native species of Iran and consists of about 200 species of herbaceous flowering plants, commonly known as ‘figwort’. Many iridoid glycosides have been found in the Scrophularia genus. In this research work the methanolic extracts of Scrophularia umbrosa (S. umbrosa) rhizome was studied. For this first methanolic extract of rhizome powder was subjected to the HPLC in which two materials was detected in 220 nm and was separated. In continue 1D and 2D NMR technique has been employed for the identification of the active components. finally phytochemical investigations of the S. umbrosa rhizome indicate the presence of two new acetylated iridoid glycosides, 6- O [2', 3'diacyl-4'-O-Trans-cynamoyl]-α-L-rhamnopyranosyl-aucubin and 6- O [2', 3’ diacyl-4'-O-Trans-p-methoxycinamoyl]-α-L-rhamnopyranosyl-aucubin. HNMR and CNMR were the main apparatuse to identification of these compounds which has been shown medicinal property, in rhizome of Scrophularia umbrosa.