The Effectiveness of Concentrated Growth Factor (CGF) in Facial Nerve Crush Injury

Abstract

Abstract Objectives This study aims to evaluate the effect of concentrated growth factor (CGF) on regeneration of facial nerve after crush injury. Materials and Methods A total of 14 rats were randomized into 2 groups.Control group (CG) (n = 7) received crush injury to the right facial nerve.CGF group (CGFG) (n = 7) received crush injury to the right facial nerve and consentrated growth factor prepared from their own blood thereafter.Left facial nerves were used for functional comparison.Nerve function was evaluated with whisker movements and electromyography.Histologic properties were evaluated with Hematoxylene-Eosin and Masson-trichrome staining;immunohistochemical properties were evaluated with degeneration markers Neurofilament-H and Anti-Tau. Results In CGFG,whisker functions both started earlier to recover and recovered more quickly compared with CG.CG showed significantly prolonged latency and reduced amplitudes in the first week compared with CGFG (p < 0.05).Recordings of 4th week latency and amplitudes were similar to the preoperative period in CGFG (p > 0.05);whereas recordings of same week were significantly worse in CG (p < 0.05).Edema and fibrosis were also more pronounced in CG compared with CGFG.Neurofilament-H and Anti-Tau significantly high in CG (p < 0.05). Conclusion Concentrated growth factor promotes recovery in facial crush injury.It may prove as a cost-effective,practical and effective treatment choice in peripheral nerve injury. Clinical Relevance: This study aims to evaluate the effect of concentrated growth factor (CGF) on regeneration of facial nerve after crush injury.