In vitro effects of the combination of serotonin, selenium, zinc, vitamin D and E supplementation on human sperm motility

Abstract

Abstract Background Infertility affects 15% of all couples worldwide. According to recent research, 50% of cases of infertility are solely due to male factors. A decrease in motility is directly related to infertility. The purpose of this study was to see how sperm motility was affected by the combination of serotonin (5-HT), selenium (Se), zinc (Zn), vitamin D, and vitamin E supplementation. Methods and results Semen samples were incubated for 75 minutes at 37°C in media containing varying concentrations of 5-HT, Se, Zn, vitamin D, and E. Sperm concentration and motility were assessed at the end of the incubation. Calculations were made for each supplement to determine the proportions of fast progressive (+ 4), slow progressive (+ 3), non-progressive (+ 2), and immotile (+ 1) sperm cells. There was a significant improvement in fast progressive motility when compared to the control groups. 5-HT (200 µM), Se (2 µg), Zn (10 µg), vitamin D (100 nM), and vitamin E (2 mmol) concentrations have also been shown to increase fast progressive sperm motility. Three different mixtures were tested for their effects on sperm motility, and the one with the highest serotonin content was also found to have higher sperm motility than the others. While the mean + 4 sperm motility in the control group was 5.26%, it increased to 16.45% in the first mixture. Conclusion Sperm motility is improved by incubating with 5-HT, Se, Zn, Vitamin D, and E. These molecules may enhance the success of assisted reproduction techniques when present in sperm preparation media.