Impact of PGF2α and Estrogen based short term estrus synchronization protocols on estrus expression, luteal profile and conception rate in cyclic Murrah buffaloes

Abstract

Abstract The study was conducted in 94 cyclic Murrah buffaloes with aim to develop a short-term estrus synchronization protocol consisting of Estradiol benzoate after PGF2α and evaluation of estrus response, luteal profile and conception rae. The buffaloes were divided into two groups with Group-I (n = 40) received PGF2α day 0 and AI was performed twice at 72 and 84h of PGF2α injection while, in Group-II (n = 54): Estradiol benzoate (1mg) 24h of PGF2α injection and AI was performed twice at 72 and 84h of PGF2α injection. Blood sampling for hormonal estimation and USG was carried out before the treatment (day 0), day 1, at the time of first-AI, and day 5 and 12 post-AI. The buffaloes showing intense estrus response were significantly (P < 0.05) greater in Group-II (33.33%; 18/54) than Group-I (7.50%; 3/40). FSCR (%) was higher in Group-II (33.33%; P > 0.05) than Group-I (27.5%). Plasma estrogen concentration of Group-II was significantly (P < 0.05) higher than Group-I on day of AI. The plasma estrogen, IGF-1 concentration was greater (P < 0.05) in pregnant than their non-pregnant counterparts in both groups. The plasma P4 (ng/ml) concentration of pregnant buffaloes on day 5 and 12 post-AI was significantly (P < 0.05) greater than non-pregnant buffalo in both groups. So, it was concluded that short term estrus synchronization protocol with administration of Estradiol benzoate 24h after PGF2α improved the estrus response, plasma estrogen concentration at AI with better post-ovulation luteal profile in cyclic Murrah buffaloes.