Ion Mobility-Tandem Mass Spectrometry of Mucin-type O-Glycans

Author:

Pagel Kevin1ORCID,Bechtella Leïla1,Chunsheng Jin2ORCID,Fentker Kerstin3ORCID,Ertürk Güney1,Safferthal Marc1ORCID,Polewski Łukasz1,Götze Michael4,Graeber Simon5ORCID,Struwe Weston6,Mall Marcus7ORCID,Mertins Philipp8ORCID,Karlsson Niclas9

Affiliation:

1. Freie Universität Berlin

2. University of Gothenburg

3. Max Delbrück Center for Molecular Medicine

4. ETH Zurich

5. Charité - Universitätsmedizin Berlin

6. University of Oxford

7. Charité – Universitätsmedizin Berlin

8. Max Delbrück Center for Molecular Medicine in the Helmholtz Association

9. Oslo Metropolitan University

Abstract

Abstract The dense O-glycosylation of mucins plays an important role in the defensive properties of the mucus hydrogel. Aberrant glycosylation is often correlated with inflammation and pathology such as COPD, cancer, and Crohn’s disease. The inherent complexity of glycans and the diversity in the O-core structure constitute fundamental challenges for the analysis of mucin-type O-glycans. Due to coexistence of multiple isomers, multidimensional workflows such as LC-MS are required. To separate the highly polar carbohydrates, porous graphitized carbon is often used as a stationary phase. However, LC-MS workflows are time-consuming and lack reproducibility. Here we present a rapid alternative for separating and identifying O-glycans released from mucins based on trapped ion mobility mass spectrometry. Compared to established LC-MS, the acquisition time is drastically reduced from an hour to two minutes. To test the validity, the developed workflow was applied to sputum samples from cystic fibrosis patients to map O-glycosylation features associated with disease.

Publisher

Research Square Platform LLC

Reference66 articles.

1. Mucin structure, aggregation, physiological functions and biomedical applications;Bansil R;Curr Opin Colloid Interface Sci,2006

2. Host mucin glycosylation plays a role in bacterial adhesion in lungs of individuals with cystic fibrosis;Venkatakrishnan V;Expert Rev Respir Med,2013

3. Glycans and glycoproteins as specific biomarkers for cancer;Kailemia MJ;Anal Bioanal Chem,2017

4. Mass Spectrometry-Based Techniques to Elucidate the Sugar Code;Grabarics M;Chem Rev,2022

5. Next Generation O-Linked Glycomics;Karlsson NG;Trends in Glycoscience and Glycotechnology,2017

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