Preparation, Investigation, and Comparison of Nickel-Agarose Coated Adsorbent Immobilized by Cibacron Blue Dye Ligand

Abstract

Abstract In this study, we looking forward to a method to obtain the best formula to make an agarose-coated adsorbent. Determining the type and number of experiments was designed by Taguchi software and our variables included stirring speed, agarose concentration, nickel dosage, and surfactant percentage. 2@NAC-CB particles have been prepared by three phase emulsion method. The results showed that the particle size decreases with increasing stirring speed, despite increasing the initial concentration of the surfactant led to a decrease in the size of the adsorbent particles. However, by increasing the surfactant concentration and stirring speed, the best result was obtained at 4% surfactant concentration and 950 rpm speed. The behavior of agarose-coated adsorbent in different conditions was calculated by measuring the bed expansion coefficient and the axial dispersion coefficient for the slurry phase when measured in a standard coupling. Also, the agarose coating of the metal core has been investigated to produce different geometries in the construction of suitable adsorbents for the purification of numerous protein products. The final result showed that the production of spherical porous adsorbent nanoparticles from natural protein in the above method can be widely used for protein purification in the industry.