Silver nitrate (AgNO3) and Pyrazinamide (PZA) as ethylene inhibitors ameliorate in vitro salt tolerance of tomato (Lycopersicon esculentum L) plant.

Abstract

Abstract The purpose of this research was to find out how in vitro culture of tomato explants respond to salt stress by silver nitrate (AgNO3) and pyrazinamide (PZA) as ethylene inhibitors. Tomato seedlings were grown on MS medium containing NaCl (0, 100, 150 mM) and supplemented with AgNO3 (0, 2, 4, mg. L-1) and pyrazinamide (PZA) (0, 2, 4,mg. L-1) to study growth parameters and antioxidant enzymes responses. Salt-stressed plants showed limited growth and a significant decrease in fresh and dry weight. Salinity accelerated oxidative damage by increasing hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels in tomato leaves. In the present of AgNO3 and PZA, in vitro grown tomato plants increased fresh and dry weight total chlorophyll, carotenoid in particular at 4 mg. L-1 AgNO3 and 4 mg. L-1 PZA. Moreover, silver nitrate and PZA reduced H2O2 and MDA contents and oxidative damage by enhancing antioxidant enzyme activity, including catalase, superoxide dismutase, and ascorbate peroxidase under salt stress. According to our findings, AgNO3 and PZA improved in vitro salinity tolerance of tomato plant by interfering ethylene action or ethylene generation and increasing biochemical responses.