Pseudoalteromonas insulae sp. nov., isolated from a tidal flat

Abstract

Abstract A Gram-stain-negative, aerobic, non-spore-forming, motile by single polar flagellum and ovoid or rod-shaped bacterial strain, designated BDTF-M6T, was isolated from tidal flat sediment collected from the Yellow Sea, Republic of Korea. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain BDTF-M6T fell within the clade comprising the type strains of Pseudoalteromonas species. Strain BDTF-M6T exhibited the highest 16S rRNA gene sequence similarity value (98.3%) to the type strain of P. caenipelagi and sequence similarities of less than 97.9% to the type strains of the other Pseudoalteromonas species. The DNA G+C content of strain BDTF-M6T from genomic sequence data was 50.0%. The ANI and dDDH values between strain BDTF-M6T and the type strains of forty-four Pseudoalteromonas species were less than 70.3 and 22.6%, respectively. Strain BDTF-M6T contained Q-8 as the predominant ubiquinone and C16:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and C18:1 ω7c as the major fatty acids. The major polar lipids of strain BDTF-M6T were phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and one unidentified glycolipid. Distinguished phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain BDTF-M6T is separated from recognized Pseudoalteromonas species. On the basis of the data presented, strain BDTF-M6T is considered to represent a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas insulae sp. nov. is proposed. The type strain is BDTF-M6T (= KACC 22179T = NBRC 115118T).