Investigation of the Prognostic Significance of Immunohistochemical Expression of p16 and p27 in Bladder Tumors

Abstract

Abstract Objective:The most important prognostic factors in Urothelial tumors of the bladder are pathological stage and histological grade. In this study, p16 and p27 immunohistochemical expressions were investigated and it was aimed to examine their relationship with clinicopathological parameters. Materials and Methods: In this study, 112 patients were diagnosed with bladder urothelial tumor in the Pathology Department of Kafkas University Faculty of Medicine between 2015 and 2020, were sent as transurethral bladder tumor resection material, and had clinicopathological information, paraffin blocks and hematoxylin-eosin (H&E) stained sections in the archive. case was included. P16 and p27 immunohistochemical staining was applied to the sections obtained from paraffin blocks. Results: The data obtained showed that the P16 IHC staining scores of the protein in the cytoplasm and nucleus did not differ statistically significantly according to the gender factor in the patients included in the study. However, it is noteworthy that the protein staining frequency distribution in female patients is high, especially in weak and medium staining, while in male patients, strong staining has the highest frequency distribution (31.0%). Although the IHC staining score frequency distributions of the P27 protein are similar, the statistical analysis showed that the distributions of this protein differ according to gender to the level of p < 0.05; This difference is similar to the nuclear distribution of P16 protein; male patients mostly had strong P27 nuclear staining (35.7%), while female patients showed strong staining at low frequencies (7.1%). Conclusion: Many parameters are used to predict prognosis in bladder tumors. As a result, it is thought that p16, p27 immunoreactivities may be helpful in predicting the clinicopathological behavior of bladder tumors, and studies involving more cases are needed.