The Relationship between AQP1 Expression and Severity of Hydrocephalus in Rattus Norvegicus Strain Sprague-Dawley Rats

Abstract

Abstract Purpose: We evaluate the relationship between the severity of hydrocephalus and AQP­1 levels. Methods: This research was conducted in an experimental research design. The research subjects were Rattus Norvegicus rats of the Sprague-Dawley strain which were injected with kaolin to create a hydrocephalus model. The study included 24 rats in total, which were divided into four groups of six each: the control group, and the hydrocephalus induction group on day 7, day 14, and day 21. AQP1 expression was then observed histopathologically by immunohistochemical staining and counted semi-quantitatively. The collected data were then compared and analyzed using SPSS software version 24. Results: The average AQP1 expression increased with observation time in the hydrocephalus rat model on the 7th, 14th, and 21st days. The ANOVA test showed a significant difference between the four study groups (p=0.001). The correlation between the expression of AQP1 levels and the severity of hydrocephalus showed a statistically significant difference (p=0.000). The results showed an increase in the expression of AQP1 levels in the sub-ventricle zone with the higher severity of hydrocephalus. Conclusion: The severity of hydrocephalus and AQP1 levels are correlated, with the latter being higher the more severe the degree of hydrocephalus.