Hypertonic saline induces inflammation in human macrophages through the NLRP1 inflammasome

Author:

Sposito Francesca1ORCID,Northey Sarah,Charras Amandine,McNamara Paul,Hedrich Christian2

Affiliation:

1. University of Liverpool

2. Liverpool

Abstract

Abstract Nebulized hypertonic saline (3–7%) is commonly used to increase mucociliary clearance in patients with chronic airway disease and/or virus infections. However, altered salt concentrations may contribute to inflammatory responses. The aim of this study was to investigate whether 500mM NaCl (3%) triggers inflammation in human macrophages and identify the molecular mechanisms involved. NaCl induced pyroptosis, IL-1β, IL-18 and ASC speck release was measured in primary human monocyte-derived macrophages. Treatment with the recombinant IL-1 receptor antagonist anakinra or the NLRP3 inhibitor MCC950 did not affect NaCl-mediated inflammasome assembly. Knock-down of NLRP1 expression, but not of NLRP3 and NLRC4, reduced NaCl induced pyroptosis, proinflammatory cytokine and ASC speck release from human THP-1-derived macrophages. Data from this study suggest that 3% NaCl induced inflammatory responses in human macrophages depend on NLRP1 and inflammasome assembly. Targeting inflammation in addition to inhalation with hypertonic saline may benefit patients with inflammatory airway disease.

Publisher

Research Square Platform LLC

Reference20 articles.

1. Inhaled hypertonic saline in preschool children with cystic fibrosis (SHIP): a multicentre, randomised, double-blind, placebo-controlled trial;Ratjen F;Lancet Respir Med,2019

2. Hypertonic Saline/Epinephrine Treatment in Hospitalized Infants with Viral Bronchiolitis Reduces Hospitalization Stay: 2 Years Experience;Tal G,2006

3. Auger KA, Parker MW, Huang B. New Method, Same Answer: We Do Not Know if Hypertonic Saline Helps Bronchiolitis. Pediatrics. 2018 Sep 1;142(3):e20181868.

4. SABRE: a multicentre randomised control trial of nebulised hypertonic saline in infants hospitalised with acute bronchiolitis;Everard ML;Thorax.,2014

5. Harrison W, Angoulvant F, House S, Gajdos V, Ralston SL. Hypertonic Saline in Bronchiolitis and Type I Error: A Trial Sequential Analysis. Pediatrics. 2018 Sep 1;142(3):e20181144.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3