Investigation of the Role of Molecules in DNA Repair Process in Coronary Artery Patients

Abstract

Abstract Background The role of DNA damage in the progression of coronary artery disease (CAD) is widely recognized. Among the factors that determine the extent of DNA damage, genetic factors may be one of the determining factors in the pathogenesis of CAD. Methods & Results In our research, we investigated the expression levels of BRCA1 and PARP1, which are involved in the DNA repair process, as well as the regulators of gene expression for these molecules, namely miR-21-5p, miR-193b-3p, and miR-484, in lymphocyte samples collected from 55 patients with CAD and 55 healthy controls. The fold changes of BRCA1, PARP1, miR-21-5p, miR-193b-3p and miR-484 expression levels in the patient group, as determined by the 2−ΔΔCT calculation, were found to be 0.353, 0.332, 0.734, 0.876, and 1.231, respectively. In the patient group, a statistically significant negative correlation was observed only between PARP1 and miR-21 (r=-0.66, p=0.0001). ConclusionsThe expression levels in molecules related to the DNA repair systems of CAD patients are clearly related to the pathogenesis of the disease, and considering this situation, measures to be taken would be beneficial.