Neuroprotective and memory enhancing effect of Nose to Brain formulation of 6- Hydroxyflavone in icv-STZ-induced Alzheimer's disease mouse model

Abstract

Abstract Alzheimer's disease characterized by memory and cognitive impairments as well as neurobehavioral abnormalities. Streptozotocin (STZ) can induce a condition analogous to alzheimer's disease by promoting the accumulation of plaques and neurofibrillary tangles, resulted in progressive cognitive decline. 6-hydroxyflavone (6HOF) is a flavone that occurs naturally in the foliage of Barleria prionitis, an Acanthaceae plant species native to India. The purpose of this study was to examine the effects of intranasal formulation of 6-Hydroxyflavon (6HOF-NTB) in mice that had been administered STZ (3mg/kg of icv) unilaterally. Neurodegeneration, and oxidative stress were used to evaluate the impact on working memory and neuroprotection. Memory impairment was developed by intracerebrovascular (icv) injections of streptozotocin (3mg/kg). Upon 21-day administration of 6HOF-NTB and Oral administrations of 6-HOF the behavioral parameter was studied using Morris water maze, novel object recognitions test, Y-maze, EPM etc. and the level of acetylcholinesterase and antioxidants was measured in brain homogenate.In a mouse model of a condition like Alzheimer's, demonstrated significant effect than the STZ-treated group. The 6HOF-NTB (1 mg/kg) increased transfer latency in the EPM, spontaneous alternation in the Y-maze, discrimination index, and novelty preference in the NOR. In addition, in the MWM, the number of platform crossings and time spent in the target quadrants increased, indicating an improvement in working spatial memory. In addition, the intranasal formulation inhibited oxidative stress and acetylcholinesterase levels in the brain, thereby reducing neurodegeneration. In the STZ induced memory impairment model in mice, the intranasal formulation of 6 HOF demonstrated desirable effects on cognitive functions and neuroprotection.